Human Opacity-Associated Proteins (OPAs) ELISA Kit – Important Notes for Sample Handling
Before collecting any biological samples, it is essential to have a clear and well-structured plan in place. Ensure that the target analytes are stable under the conditions you intend to use. We strongly recommend using fresh specimens whenever possible, as this helps maintain the integrity of the proteins being tested.
If testing can be done on the same day after collection, store the samples at 4°C immediately. For long-term storage or if sampling needs to be done periodically, it is crucial to handle the samples properly. Once collected, aliquot the specimens promptly and store them at -20°C or, preferably, at -70°C. Avoid repeated freeze-thaw cycles, as temperature fluctuations between the ice chamber and room temperature can lead to protein degradation, which may negatively impact the accuracy and reliability of your results.
It is important to note that improper handling of samples can significantly affect the performance of the ELISA assay. Therefore, customers who are planning to collect and test samples must consult with our sales representatives before proceeding. Additionally, we recommend reaching out to our technical support team for detailed guidance on sample preparation, storage, and handling procedures. This ensures that all steps are followed correctly, maximizing the quality and consistency of your experimental data.
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