**Human Prolactin (PRL) ELISA Kit Instruction Manual**
**Kit Specifications:**
This ELISA kit is available in 48-well or 96-well configurations. The standard dilution is 1.5 mL × 1 bottle. The enzyme standard reagent is 3 mL × 1 bottle (for 48-well) or 6 mL × 1 bottle (for 96-well). This reagent is for research use only.
**Standard Curve Preparation:**
The concentration of the standard is plotted on the x-axis, and the OD value is on the y-axis. A standard curve is drawn using coordinate paper, and the sample concentration is determined by matching its OD value to the curve. Alternatively, a linear regression equation can be calculated from the standard data, and the sample OD value is substituted into the equation to determine the actual concentration, which is then multiplied by the dilution factor.
**Kit Composition:**
- Sealing film: 2 pieces (48) / 2 pieces (96)
- Instructions: 1 part
- Standard: 2700 ng/L, 0.5 mL × 1 bottle
- Enzyme standard: 1×48 / 1×96
- Sample diluent: 3 mL × 1 bottle (48) / 6 mL × 1 bottle (96)
- Developer A: 3 mL × 1 bottle (48) / 6 mL × 1 bottle (96)
- Chromogen B: 3 mL × 1 bottle (48) / 6 mL × 1 bottle (96)
- Wash solution: 3 mL × 1 bottle (48) / 6 mL × 1 bottle (96)
- Concentrated wash solution: 20 mL × 20 times × 1 bottle (48) / 20 mL × 30 times × 1 bottle (96)
**Principle of Operation:**
This kit uses the double-antibody sandwich method to detect human prolactin (PRL) E levels in the sample. The microplate is pre-coated with purified anti-PRL E antibody. After adding the sample and HRP-labeled secondary antibody, a complex forms. After washing, TMB substrate is added, producing a color change that is proportional to the PRL E concentration. The reaction is stopped, and the absorbance at 450 nm is measured to calculate the concentration based on the standard curve.
**Purpose:**
This kit is designed to quantify anti-endometrial antibodies (EMAb) in human serum, plasma, urine, and other biological fluids.
**Storage Conditions & Expiration:**
- Storage: 2–8°C
- Shelf Life: 6 months
**Sample Preparation Guidelines:**
1. **Serum:** Allow blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes.
2. **Plasma:** Use EDTA or sodium citrate as anticoagulant, mix for 10–20 minutes, then centrifuge.
3. **Urine:** Collect in a sterile tube and centrifuge.
4. **Cell culture supernatant:** Centrifuge after collection; for intracellular components, lyse cells and centrifuge again.
5. **Tissue specimens:** Homogenize in PBS, centrifuge, and collect supernatant.
6. **General:** Process samples as soon as possible. Store at -20°C if not tested immediately. Avoid repeated freeze-thaw cycles.
7. **Avoid NaN3**, as it inhibits HRP activity.
**Operating Steps:**
1. **Standard Dilution:** Prepare serial dilutions in 10 wells.
2. **Loading:** Add 40 μL of sample diluent and 10 μL of sample to each well.
3. **Incubation:** Seal and incubate at 37°C for 30 minutes.
4. **Washing:** Use diluted washing solution and wash 5 times.
5. **Enzyme Addition:** Add 50 μL of enzyme conjugate to each well.
6. **Second Incubation:** Repeat incubation at 37°C for 30 minutes.
7. **Color Development:** Add 50 μL of TMB A and B, incubate for 15 minutes at 37°C.
8. **Stop Reaction:** Add 50 μL of stop solution.
9. **Measurement:** Read OD at 450 nm within 15 minutes.
**Notes:**
- Equilibrate the kit at room temperature before use.
- Avoid cross-contamination by using a new sealing film for each test.
- Keep substrates away from light.
- Always prepare a standard curve and use duplicate wells for accuracy.
- Follow instructions strictly and validate results with a microplate reader.
- Treat all waste materials as biohazardous.
- Do not mix reagents from different batches.
- In case of conflict, the English manual takes precedence.
**Performance:**
- Linear regression correlation coefficient R ≥ 0.95
- Intra-batch and inter-batch variation < 9% and 11%, respectively
- Detection range: 0.2 IU/L – 6 IU/L
**Service Commitment:**
- Free technical support during working hours
- Free sample testing services available upon request
- Delivery within payment period
This detailed guide ensures accurate and reliable detection of PRL E levels in various biological samples. Always follow the protocol carefully for optimal results.
Similar Microfiber Products
In terms of car maintenance, microfiber towels can easily remove dirt and water stains from the surface of the car without scratching the paint, and can also be used to wipe the dashboard and seats in the car.
In the Mulberry Bath hotel, microfiber towels can be used to wipe the body and absorb water, with a comfortable soft feel without irritating the skin.
In the beauty and hair industry, microfiber towels can be used to wipe the face and hair, with the advantages of thorough cleaning and fast absorption of water, but also can be used to wipe beauty instruments and hair tools.
In terms of sporting goods, microfiber towels can be used to wipe sweat and absorb water for a quick dry effect, but also can be used to wipe sports equipment and shoes, etc.
In terms of daily necessities, microfiber towels can be used to wipe furniture, electrical appliances and floors, etc., with decontamination and clean, absorbent fast characteristics, but also can be used to wipe pet and children and other sensitive skin.
Microfiber cloth,Microfiber towels, Microfiber cloth for cleaning, Microfiber fabric
NINGBO JENNY IMPORT & EXPORT CO.,LTD , https://www.jenny-china.com