**Vaspin Kit – Visceral Fat-Specific Serine Protease Inhibitor**
The Vaspin Kit is designed for the quantitative determination of visceral fat-specific serine protease inhibitor (vaspin) in human serum, plasma, and other biological fluid samples. This assay is based on the double-antibody sandwich immunoassay principle, ensuring high specificity and sensitivity for vaspin detection.
The procedure involves coating a microplate with a purified monoclonal antibody specific to human vaspin. After incubation with the sample, vaspin from the sample binds to the immobilized antibody. A horseradish peroxidase (HRP)-labeled secondary antibody is then added, forming a complex of antibody-antigen-enzyme-labeled antibody. Following a washing step to remove unbound components, a TMB substrate solution is introduced. Under the catalytic action of HRP, TMB turns blue and then changes to yellow upon acid termination. The intensity of the color is directly proportional to the concentration of vaspin in the sample.
The optical density (OD) is measured at 450 nm using a microplate reader. By comparing the OD values to a standard curve generated from known concentrations of vaspin, the exact level of vaspin in the sample can be accurately determined.
**Kit Components:**
- Microtiter Plate (coated with anti-vaspin antibody): 1 × 48 wells / 1 × 96 wells
- Standard: 36 ng/ml, 0.5 ml × 1 bottle
- Standard Diluent: 1.5 ml × 1 bottle
- Enzyme-Labeled Antibody: 3 ml × 1 bottle
- Sample Diluent: 3 ml × 1 bottle
- TMB Substrate: 3 ml × 1 bottle
- Stop Solution: 3 ml × 1 bottle
- Wash Buffer (20×): 20 ml × 20 times / 20 ml × 30 times
- Sealing Film: 2 pieces (for 48-well) / 2 pieces (for 96-well)
- Sealing Bag: 1 piece
**Storage Instructions:**
All reagents should be stored at 2–8°C. The kit is stable for up to 12 months when stored properly.
This kit provides a reliable and efficient method for measuring vaspin levels, which may serve as a potential biomarker for metabolic disorders, obesity, and related conditions. It is ideal for research laboratories and clinical settings where accurate quantification of vaspin is required.
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