Abstract: Liquid chromatography is a separation technique that utilizes a liquid as the mobile phase to carry the sample through a stationary phase. High-performance liquid chromatography (HPLC) has become an essential tool in medicine and biochemistry, offering precise and efficient analysis of complex mixtures. Its applications include the detection of abnormal metabolites in body fluids, which plays a critical role in diagnosing metabolic disorders. HPLC is also widely used for analyzing drug components, ensuring their purity and quality during pharmaceutical development. In drug production, it serves as an important intermediate control method to monitor reaction progress and product consistency. Additionally, it helps determine the residual levels of drugs in biological systems, aiding in pharmacokinetic studies. HPLC is also employed to quantify drugs in various organs, providing valuable information on distribution and metabolism. Beyond drugs, it is used for the qualitative and quantitative analysis of biological molecules such as nucleic acids, amino acids, enzymes, sugars, and organic acids like citric acid. It is also applied in the analysis of sputum components, contributing to the understanding of respiratory diseases. Overall, HPLC remains a fundamental analytical technique with broad applications across medical and biochemical research.
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