ã€Abstractã€‘Objective: To establish a HPLC-ELSD method for the determination of spectinomycin hydrochloride. Method: Grace-Apollo C_(18) (150 mm Ã— 4.6 mm, 5 Î¼m) was used as the mobile phase, 50 mmolÂ·L -1 - ammonium acetate solution (pH 7.5) - methanol (92:8) as mobile phase The flow rate is 0.6 mlÂ·min~(-1), the spray chamber temperature is 30 Â°C, and the drift tube temperature is 70 Â°C. Atomizing gas (air) pressure: 0.4 Mpa. Results: The spectinomycin hydrochloride showed a good linear relationship (r=0.999 0) in the range of 0.101ï½ž0.504 mgÂ·ml~(-1), and the average recovery was 98.5% (RSD=1.6%, n=9). Conclusion: This method is simple, rapid, accurate and reliable, and can be applied to the determination of spectinomycin hydrochloride.
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