ELISA has a wide range of applications and is constantly expanding. In principle, ELISA can be used to detect all antigens, antibodies and haptens, and can directly quantify soluble antigens in body fluids. In practical application, it can be used for clinical diagnosis of diseases, disease monitoring, disease general survey, forensic examination, veterinary and agricultural plant disease diagnosis and verification, etc. Therefore, it is closely related to biochemistry, immunology, microbiology, pharmacology, epidemiology and infectious diseases. According to different properties of the analyte, ELISA reactions can be divided into antigen detection reactions and antibody detection reactions.
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