The Shanghai Xinzhuang Biological Safety Cabinet is designed to protect the operator, the laboratory environment, and the experimental materials from exposure to infectious agents. It is used when handling primary cultures, strains, and diagnostic specimens that may be infectious. The cabinet is specifically engineered to contain infectious aerosols and potential spills that might occur during experiments.
To ensure optimal performance, it's important to understand the technical specifications of the Shanghai Shengzhuang Biological Safety Cabinet:
1. **Operation and Maintenance**
Most biosafety cabinets are built for 24/7 operation. Continuous use helps maintain a clean lab environment by reducing dust and particle accumulation. Class A1 and A2 cabinets, which vent air into the room or through a duct, can be turned off when not in use. However, Class II B1 and B2 cabinets require constant airflow through rigid ducts to maintain air balance in the room. Before and after each session, allow the cabinet to run for at least five minutes to complete the "purification" cycle, ensuring contaminated air is properly expelled.
2. **Airflow Velocity**
The air entering the front opening of the safety cabinet should flow at approximately 0.45 m/s. This speed is highly sensitive to disturbances such as people moving nearby, open windows, or door openings. To maintain consistent airflow, the cabinet should be placed in an area with minimal foot traffic and where items are not frequently moved. Leave at least 30 cm of space on both sides and behind the cabinet for maintenance. A clearance of 30–35 cm above the unit is also recommended for accurate airflow measurement and filter replacement.
3. **Operator Technique**
Improper use of the cabinet can significantly reduce its protective effectiveness. When moving arms in and out of the front opening, do so slowly and vertically to preserve the airflow barrier. Allow about one minute for the cabinet to adjust and "sweep" the air over your hands before starting work. Always place all necessary items inside the cabinet before beginning the experiment to minimize movement in and out of the front.
4. **Item Placement**
Avoid blocking the front air intake grille with papers, instruments, or other objects. All items placed inside should be decontaminated with 70% alcohol. Use disinfectant-soaked towels to manage possible spills. Place items as close to the back wall as possible to avoid obstructing the airflow. Equipment that produces aerosols, like mixers or centrifuges, should be positioned near the rear of the cabinet. Bulky waste containers, such as those holding used pipettes or suction flasks, should be placed on one side. Always work from the clean area toward the contaminated area on the bench.
5. **UV Lamps**
UV lamps are not typically required in biosafety cabinets. If used, they should be cleaned weekly to maintain their effectiveness. During recertification, the UV light intensity is checked to ensure proper germicidal output. Never leave the UV lamp on when someone is in the room, as it can cause eye and skin damage.
6. **Open Flames**
Open flames should be avoided in biosafety cabinets due to their potential to disrupt airflow and pose a risk when working with volatile or flammable substances. Instead, use a micro-burner or electric heater for sterilizing loops.
7. **Spill Management**
Laboratories must have clear procedures for spill management. All personnel should be familiar with these protocols. In case of a spill, clean it immediately once the cabinet is operational. Use effective disinfectants and minimize aerosol generation. All materials that came into contact with the spill must be sterilized or autoclaved.
8. **Cabinet Evaluation**
Regular assessments of the safety cabinet’s effectiveness should include checking its structural integrity and airflow performance.
Shanghai Xinzhuang specializes in manufacturing high-quality biological safety cabinets and other lab equipment. With advanced technology and strict quality control, we offer reliable solutions for laboratories worldwide. For more information or to place an order, feel free to reach out.
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